Establishment of Microfluidic Chip Method for Rapid Screening of <i>Tobacco Rattle Virus</i>

Tobacco rattle virus (TRV), member of the genus Tobravirus, is an important plant pathogen with a wide host range beyond any known viruses. It able to be transmitted by nematodes genera Trichodorus and Paratrichodorus (Trichodoridae). In this paper, rapid screening method was established for inspection TRV in seeds or other materials, combining microfluidic chip technique fluorescence detection system. The particular region (located at 496-995) strain (accession number: KP100069.1) adopted primer design, establishment improvement. Through analysis, proved specific only detecting sample infected TRV, while 7 leaf materials Tomato spot wilt (TSWV), ringspot (TRSV), Cucumber mosaic (CMV), Lily symptomless (LSV), (LMV), (ToRSV), Prunus necrotic (PNRSV), respectively, showed negative results. Sensitivity tests represented limit as low 1.00×102 copies/μL which normal PCR assays. one word, based analyzing platforms hold high promises enable high-throughput high-precision less consumption, fast detection, simple operation, multi-functional integration, small size, multiplex portability, thus promoting development biosecurity diagnostics.